NHE3/SLC9A3 [p Ser552] Antibody (14D5) NB110-81529 from Novus Biologicals, a Bio-Techne Brand

Product Specs
ItemNHE3/SLC9A3 [p Ser552] Antibody (14D5)
CompanyNovus Biologicals, a Bio-Techne Brand
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberNB110-81529
ImmunogenSynthetic peptide made to a region surrounding the Serine 552 portion of rat NHE3. [UniProt# P26433]
Concentration2 mg/ml
ClonalityMonoclonal
Conjugate/TagUnconjugated
TargetNHE3/SLC9A3
Clone14D5
ApplicationsWestern Blot, Simple Western, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated
ReactivityHuman, Mouse, Rat, Porcine, Opossum, Primate, Rabbit
FormatTris-Glycine and 0.15M NaCl
Quantity0.1 ml (also 0.025 ml)
HostMouse
IsotypeIgG2a
Modificationsp Ser552
Brand/TrademarkNovus Biologicals, a Bio-Techne Brand
PurityProtein G purified
Molecular Weight85 kDa
Research AreaLipid and Metabolism, Plasma Membrane Markers, Signal Transduction
NCBI Full Gene Namesolute carrier family 9 member A3
NCBI Gene AliasesDIAR8, NHE-3, NHE3
ValidationKnockout Validated
Antibody TypePrimary Antibodies
StorageStore at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles

Specifications/Features

Available conjugates: Unconjugated

Specificity: This antibody detects NHE3 posphorylated at Serine 552, and does not cross-react with the non-phosphopeptide

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Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: http://www.novusbio.com

(1) The AE4 transporter mediates kidney acid-base sensingNature communicationsMay 26, 2023Vitzthum H, Koch M, Eckermann L, Svendsen SL, Berg P, Hübner CA, Wagner CA, Leipziger J, Meyer-Schwesinger C, Ehmke H.S995B), rabbit anti-NHE3 (WB 1:10,000, StressMarq SPC 400D), mouse anti-NHE3 Ser552P (WB 1:10,000, Novus NB110-81529 Clone 14D5), rhodamine wheat germ agglutinin (IF 1:400; Vector), and Hoechst (1:1000; Molecular Probes).

(2) Segment-specific effects of resveratrol on porcine small intestinal dipeptide absorption depend on the mucosal pH and are due to different mechanisms: potential roles of different transport proteins and protein kinasesThe Journal of nutritional biochemistryNovember 1, 2020Klinger S.

(3) Fructose increases the activity of sodium hydrogen exchanger in renal proximal tubules that is dependent on ketohexokinaseThe Journal of nutritional biochemistrySeptember 1, 2019Hayasaki T, Ishimoto T, Doke T, Hirayama A, Soga T, Furuhashi K, Kato N, Kosugi T, Tsuboi N, Lanaspa MA, et al.

(4) Podocyte Injury Augments Intrarenal Angiotensin II Generation and Sodium Retention in a Megalin-Dependent MannerHypertension (Dallas, Tex. : 1979)September 1, 2019Koizumi M, Ueda K, Niimura F, Nishiyama A, Yanagita M, Saito A, Pastan I, Fujita T, Fukagawa M, Matsusaka T.

(5) Elevated glucose concentration in culture media decreases membrane trafficking of SGLT2 in LLC-PK₁ cells via a cAMP/PKA-dependent pathwayAmerican journal of physiology. Cell physiologyJune 1, 2019Sunilkumar S, Ford SM.acquired from Cell Signaling Technology (Danvers, MA). Na⫹/H⫹ exchanger 3 (NHE3) antibody (catalog no. NB110-81529) (28) was obtained from Novus Biological (Littleton, CO). FITCconjugated anti-mouse IgG (catalog no.

(6) Unmasking a sustained negative effect of SGLT2 inhibition on body fluid volume in the ratAmerican journal of physiology. Renal physiologySeptember 1, 2018Masuda T, Watanabe Y, Fukuda K, Watanabe M, Onishi A, Ohara K, Imai T, Koepsell H, Muto S, Vallon V, et al.sc-53961, Santa Cruz Biotechnology, Santa Cruz, CA) (31), phosphorylated (S552) NHE3 (dilution 1:1,000, NB110 – 81529, Novus Biologicals, Littleton, CO) (31), aquaporin 1 (AQP1; dilution 1:1,000, AB2219, Merck Millipore)

(7) Resveratrol Inhibits Porcine Intestinal Glucose and Alanine Transport: Potential Roles of Na⁺/K⁺-ATPase Activity, Protein Kinase A, AMP-Activated Protein Kinase and the Association of Selected Nutrient Transport Proteins with Detergent Resistant MembranesNutrientsMarch 3, 2018Klinger S, Breves G.antibodies: rabbit-anti-NHE3 (sc-16103-R, Santa Cruz Biotechnology, Dallas, TX, USA), mouse-anti-NHE3 Ser552 (NB110-81529, Novus Biologicals, Littleton, CO, USA, mouse-anti-NHE3 Ser605 (NB110-74678SS, Novus Biologicals); PEPT1,

(8) Renal tubular NHE3 is required in the maintenance of water and sodium chloride homeostasisKidney internationalAugust 1, 2017Fenton RA, Poulsen SB, de la Mora Chavez S, Soleimani M, Dominguez Rieg JA, Rieg T.

(9) NHE3 phosphorylation via PKCη marks the polarity and orientation of directionally migrating cellsCellular and molecular life sciences : CMLSDecember 1, 2014Özkucur N, Song B, Bola S, Zhang L, Reid B, Fu G, Funk RHW, Zhao M.

(10) Phospho-NHE3 forms membrane patches and interacts with beta-actin to sense and maintain constant direction during cell migrationExperimental cell researchMay 15, 2014Perike S, Özkucur N, Sharma P, Staroske W, Bläsche R, Barth K, Funk RH.

(11) Gastrin induces sodium-hydrogen exchanger 3 phosphorylation and mTOR activation via a phosphoinositide 3-kinase-/protein kinase C-dependent but AKT-independent pathway in renal proximal tubule cells derived from a normotensive male humanEndocrinologyFebruary 1, 2013Liu T, Jose PA.

(12) Natriuretic effect by exendin-4, but not the DPP-4 inhibitor alogliptin, is mediated via the GLP-1 receptor and preserved in obese type 2 diabetic miceAmerican journal of physiology. Renal physiologyOctober 1, 2012Rieg T, Gerasimova M, Murray F, Masuda T, Tang T, Rose M, Drucker DJ, Vallon V.

(13) Persistent directional cell migration requires ion transport proteins as direction sensors and membrane potential differences in order to maintain directednessBMC cell biologyJanuary 22, 2011Ozkucur N, Perike S, Sharma P, Funk RH.

(14) NHE3 function and phosphorylation are regulated by a calyculin A-sensitive phosphataseAmerican journal of physiology. Renal physiologyMarch 1, 2010Dynia DW, Steinmetz AG, Kocinsky HS.

(15) NHE3 phosphorylation at serines 552 and 605 does not directly affect NHE3 activityAmerican journal of physiology. Renal physiologyJuly 1, 2007Kocinsky HS, Dynia DW, Wang T, Aronson PS.

(16) Use of phospho-specific antibodies to determine the phosphorylation of endogenous Na+/H+ exchanger NHE3 at PKA consensus sitesAmerican journal of physiology. Renal physiologyAugust 1, 2005Kocinsky HS, Girardi AC, Biemesderfer D, Nguyen T, Mentone S, Orlowski J, Aronson PS.

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NHE3/SLC9A3 [p Ser552] Antibody (14D5) from Novus Biologicals, a Bio-Techne Brand

Specifications/Features

Novus Biologicals, a Bio-Techne Brand

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NHE3/SLC9A3 [p Ser552] Antibody (14D5) from Novus Biologicals, a Bio-Techne Brand

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